首页> 外文OA文献 >Utility of WT-1, p63, MOC31, mesothelin, and cytokeratin (K903 and CK5/6) immunostains in differentiating adenocarcinoma, squamous cell carcinoma, and malignant mesothelioma in effusions
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Utility of WT-1, p63, MOC31, mesothelin, and cytokeratin (K903 and CK5/6) immunostains in differentiating adenocarcinoma, squamous cell carcinoma, and malignant mesothelioma in effusions

机译:WT-1,p63,mOC31,间皮素和细胞角蛋白(K903和CK5 / 6)免疫组化在分泌腺癌,鳞状细胞癌和恶性间皮瘤中的应用

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摘要

To distinguish carcinoma, either adenocarcinoma (ADC) or squamous cell carcinoma (SCC), and malignant mesothelioma (MM) in effusion can be a diagnostic challenge based on morphology alone. This study evaluates the utility of WT-1, p63, MOC31, mesothelin, and cytokeratin (K903 and CK5/6) immunostains in effusions when ADC and SCC of the lung are in the differential diagnosis with MM. A cohort of 43 effusions consisting of lung ADC ( N = 10), SCC ( N = 15), and MM ( N = 18, mostly (16) pleural based), was subjected to immunostains using the above mentioned antibodies. WT-1 was positive in 100% MM, 0% ADC, and 0% SCC cases while p63 was positive in 0% MM, 30% ADC, and 80% SCC cases. Stain for MOC31 was positive in 100% ADC, 67% SCC, and 35% MM cases. Similarly, mesothelin antibody stained 100% ADC, 60% SCC, and 47% MM cases. Antibodies for K903 and CK5/6 stained 100% SCC cases but fewer ADC cases (40 and 10%, respectively). In conclusion, in this cohort of mostly pleural malignant effusion, MM can be identified with positive staining for WT-1 and negative staining for p63. Conversely, negative staining with WT-1 and positive staining for p63 exclude MM. Used as part of an immunostain panel, cytokeratin markers (CK5/6 and K903) are useful in differentiating SCC from ADC when MM is already excluded, and MOC31 might have limited value in differentiating ADC from MM. A negative stain with MOC31 can exclude lung ADC. Mesothelin, on the other hand, is not useful in the differential diagnosis of ADC, SCC, and MM. Diagn. Cytopathol. 2008;36:20–25. © 2007 Wiley-Liss, Inc.
机译:要区分癌,无论是腺​​癌(ADC)还是鳞状细胞癌(SCC)和积液中的恶性间皮瘤(MM)都是仅基于形态学的诊断挑战。这项研究评估了当肺部ADC和SCC与MM进行鉴别诊断时,WT-1,p63,MOC31,间皮素和细胞角蛋白(K903和CK5 / 6)免疫染色在积液中的实用性。使用上述抗体对由肺ADC(N = 10),SCC(N = 15)和MM(N = 18,主要是(16)胸膜)组成的43例渗出液进行免疫染色。 WT-1在100%MM,0%ADC和0%SCC病例中为阳性,而p63在0%MM,30%ADC和80%SCC病例中为阳性。在100%ADC,67%SCC和35%MM病例中,MOC31的染色呈阳性。同样,间皮素抗体对100%ADC,60%SCC和47%MM病例染色。针对K903和CK5 / 6的抗体对100%的SCC病例染色,但对ADC的病例染色较少(分别为40%和10%)。综上所述,在这一主要由胸膜恶性积液组成的队列中,可以通过WT-1阳性染色和p63阴性染色来鉴定MM。相反,WT-1阴性染色和p63阳性染色排除MM。细胞角蛋白标记物(CK5 / 6和K903)用作免疫染色检测组的一部分,可用于在已经排除MM的情况下将SCC与ADC区别开来,而MOC31在将ADC与MM区别上可能价值有限。 MOC31阴性染色可排除肺ADC。另一方面,间皮素在ADC,SCC和MM的鉴别诊断中没有用。诊断细胞病。 2008; 36:20-25。 ©2007 Wiley-Liss,Inc.

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